Taurolidine, a Sulfonic Amino Acid Derivative, Induces Apoptosis and Cytotoxicity against Cervical Cell Carcinoma an In Silico and in Vitro Approach
DOI:
https://doi.org/10.54392/irjmt2546Keywords:
Taurolidine, Hirshfeld Surface Analysis, MTT Assay, Fluorescence StainingAbstract
Taurolidine, a derivative of endogenous sulfonic amino acid taurine with a chemical formula of C7H16N4O4S2 with antineoplastic, anti-inflammatory, cytotoxic, and antimicrobial characteristics, was subjected to in silico and in vitro studies. The fingerprint plots of the Hirshfeld surface analysis highlight that the O…H/H…O interaction makes a significant contribution with 57.4% of the total crystal packing. The biological interaction of taurolidine with antiapoptotic proteins Bcl-2 and Bcl-XL, characterized by binding energies of -5.54 and -6.92 kcal/mol, confirms its cytotoxic activity. The in vitro experiments were conducted against cervical carcinoma (SiHa) cells to confirm its antiproliferative activity. The MTT assay revealed that the cytotoxicity of taurolidine was observed in a dose-sensitive manner, with an IC50 (half maximal inhibitory concentration) at 204.1 µM, and induced cell cycle halt at the G0/G1 phase. The apoptotic characteristics of taurolidine-treated cells were measured using dual (AO/EtBr) and propidium iodide fluorescence staining. In addition, the mRNA expression analysis of proteins Bax and Bcl-2 confirms that taurolidine promotes apoptosis in cervical carcinoma cells.
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